Regulatory
Pveg

Part:BBa_K316001:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-12)

pVeg Constitutive promoter for Veg locus from B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using Pfu polymerase to avoid mutations


Source

PCR from existing biobrick K143053 using Pfu polymerase II